In this study, biomass and lipid production of three oleaginous microalgae (Chlorella sp. HQ, Chlorella vulgaris, and Scenedesmus sp. LX1) autotrophically cultivated in leachate of saline-alkali land from Shandong Province were investigated to explore the feasibility that utilizing saline-alkali land leachate to cultivate microalgae. Based on the comprehensive comparison, Chlorella sp. HQ was more suitable to be cultivated in leachate of saline-alkali land and the cultivation efficiency of leachate is much better than microalgal culture medium considering not only biomass but also lipid content. Hence, using leachate of saline-alkali land is promising to save cost of microalgal cultivation and synchronously the leachates can be purified.